Candesartan, candesartan cilexetil, resveratrol and their combinations change the amount of endothelial progenitor cells, cytogenetic and cytokinetic parameters in adipose tissue cell culture
AbstractCardiovascular diseases are one of the most dangerous cause of mortality in many countries. At present it is important to research new methods of treatment and prevention of these diseases and create new effective and safe drugs and their combinations to decrease the evidence of cases of cardiovascular pathology. Candesartan cilexetil is an angiotensin II receptor antagonist. It’s a precursor to the active form of candesartan. These substances have a cytotoxic effect, affecting the antioxidant system. Therefore, to prevent the cytotoxic effect is the need to use antioxidants. Resveratrol is a naturally occurring polyphenol, which has positive properties on cardiovascular system. Goal. To investigate the effect of candesartan cilexetil, candesartan and antioxidant resveratrol in different doses and combinations on the number of CD117+, CD34+, CD117+/СD34+, CD31+ stem cells, on the amount of apoptotic and micronucleated cells and cell cycle parameters in vitro. Methods. We used cell culture of adipose tissue for the experiments. After incubation with studied substances, the biological characteristics of stem cells were determined. Flow cytometry method was applied to investigate the number of CD117+, CD34+, CD117+/СD34+, CD31+ stem cells, the ratio of apoptotic cells, cells with micronuclei and parameters of cell cycle in vitro. Results. Candesartan cilexetil with resveratrol and candesartan with resveratrol were effective in the stimulation of formation of endothelial progenitor cells as compared to the control and cytostatics. Candesartan cilexetil and candesartan were cytotoxic in cell culture. Resveratrol reduced the negative effects of candesartan cilexetil/candesartan in combinations. Conclusions. Complex use of candesartan cilexetil and candesartan with natural antioxidant resveratrol showed stimulating effect on differentiation of stem cells into endothelial progenitor cells, and combinations of substances were not cytotoxic.
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